During the Introduction to Metagenomics Summer Workshop we discussed denoising amplicon sequence variants and worked through Ben Callahan’s DADA2 tutorial. During that session, I mentioned several other approaches and algorithms for denoising or clustering amplicon sequence data including UNOISE3, DeBlur and Mothur.
This post is from a tutorial demonstrating the processing of amplicon short read data in R taught as part of the Introduction to Metagenomics Summer Workshop. It provides a quick introduction some of the functionality provided by phyloseq and follows some of Paul McMurdie’s excellent tutorials.
This was a talk given as part of the UC-CCHMC introductory workshop covering the basics of microbial metagenomic sequence data processing and statistical analysis. Topics covered in this talk include an introduction to challenging features of …
This was a talk given as part of the UC-CCHMC introductory workshop covering the basics of microbial metagenomic sequence data processing and statistical analysis. Topics covered include an introduction to denoising 16S rRNA gene sequencing data, a …